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Pramod, K.
- Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Eugenol
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Authors
Affiliations
1 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062,, IN
2 Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062
3 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, IN
1 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062,, IN
2 Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062
3 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 2 (2013), Pagination: 58-61Abstract
Eugenol is an allyl chain-substituted guaiacol and finds variety of applications. Thus development of a validated UV spectrophotometric method will always be advantageous as the method is simple and rapid. The method was validated according to International Conference on Harmonisation (ICH) guidelines Q2(R1) with respect to linearity and range, precision, accuracy, detection limit (DL) and quantitation limit (QL). The detection limit (DL) and quantitation limit (QL) were determined as per the ICH guidelines and were found to be 0.82 and 2.48 μg mL-1 respectively. The method is expected to be useful in a variety of industries where eugenol finds its application.Keywords
ICH, Linearity, Range, Precision, Accuracy, Detection Limit, Quantitation Limit
References
- Pramod K, Ansari SH and Ali J. Eugenol: A natural compound with versatile pharmacological actions. Nat Prod Commun. 5 (12); 2010: 1999-2006.
- Kriegel C, Kit KM, McClements DJ, Weiss J. Nanofibers as carrier systems for antimicrobial microemulsions. II. Release characteristics and antimicrobial activity. J Appl Polym Sci. 118; 2010: 2859–2868.
- Pokharkar VB, Shekhawat PB, Dhapte VV, Mandpe LP. Development and optimization of eugenol loaded nanostructured lipid carriers for periodontal delivery. Int J Pharm Pharm Sci. 3; 2011: 138-143.
- Gomes C, Moreira RG, Castell-Perez E. Poly (DL-lactide-coglycolide) (PLGA) nanoparticles with entrapped transcinnamaldehyde and eugenol for antimicrobial delivery applications. J Food Sci. 76 (2); 2011: N16-N24.
- Shu-Ya J. 2010. Encapsulation of active substance in nanocapsules by emulsion-diffusion method [Accessed 12 February 2011]. Available from: URL: http://140.121.155.217/ seminar/19932033-1.pdf.
- Shah A, Garg A, Sairam K, Singh S. 2010. Pharmacological evaluation of eugenol loaded solid lipid nanoparticles in irritable bowel syndrome [Accessed 10 March 2011]. Available from: URL: www.scientificipca.org/paper/2009/09/25/20090925 1910570A.doc.
- Chen F, Shi Z, Neoh KG, Kang ET. Antioxidant and antibacterial activities of eugenol and carvacrol-grafted chitosan nanoparticles. Biotechnol Bioeng. 104 (1); 2009: 30-39.
- Jadhav BK, Khandelwal KR, Ketkar AR, Pisal SS. Formulation and evaluation of mucoadhesive nanocapsules containing eugenol for the treatment of periodontal diseases. Drug Dev. Ind. Pharm. 30; 2004: 195-203.
- Chaieb K, Hajlaoui H, Zmantar T, Kahla-Nakbi AB, Rouabhia M, Mahdouani K, Bakhrouf A. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytother. Res. 21; 2007: 501-506.
- ICH-Guidelines Q2(R1). Validation of Analytical Procedures: Text and Methodology. Geneva, Switzerland: 2005.
- UV Spectrophotometric Method for the Quantification of Eugenol during in Vitro Release Studies
Abstract Views :1237 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, IN
2 Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, IN
1 Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, IN
2 Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, IN
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 3 (2013), Pagination: 86-89Abstract
Polysorbate 80 (Tween 80®) is a commonly used media for solubilizing eugenol and thus could be employed as a solubilizer in the in vitro release studies of eugenol from its dosage forms. Till date no studies have been reported a validated UV spectrophotometric assay method for the estimation of eugenol in dissolution media containing Tween 80 as solubilizer. Towards this objective of quantification of eugenol efforts have been made towards the development and validation analytical method by UV spectrophotometry. The method was validated according to International Conference on Harmonisation (ICH) guidelines Q2(R1) with respect to linearity and range, precision, accuracy, detection limit (DL) and quantitation limit (QL). The detection limit and quantitation limit were determined as per the ICH guidelines and were found to be 0.62 and 1.88 μg mL-1 respectively. Thus it was confirmed that the developed method could be employed for the quantification of eugenol from 0.5% w/v aqueous Tween 80 solutions used as aqueous phase for in vitro release studies of eugenol loaded drug delivery systems.Keywords
ICH,Tween 80, Dissolution, Drug Release, ValidationReferences
- Pramod K, Ansari SH, Ali J. Eugenol: A natural compound with versatile pharmacological actions. Nat Prod Commun. 2010; 5(12): 1999-2006.
- Gomes C, Moreira RG, Castell-Perez E. Poly (DL-lactide-coglycolide) (PLGA) nanoparticles with entrapped transcinnamaldehyde and eugenol for antimicrobial delivery applications. J Food Sci. 2011; 76(2): N16-N24.
- Kriegel C, Kit KM, McClements DJ, Weiss J. Nanofibers as carrier systems for antimicrobial microemulsions. II. Release characteristics and antimicrobial activity. J Appl Polym Sci. 2010; 118: 2859–68.
- Pokharkar VB, Shekhawat PB, Dhapte VV, Mandpe LP. Development and optimization of eugenol loaded nanostructured lipid carriers for periodontal delivery. Int J Pharm Pharm Sci. 2011; 3: 138-43.
- Shu-Ya J. 2010. Encapsulation of active substance in nanocapsules by emulsion-diffusion method [Accessed 12 February 2011]. Available from: http://140.121.155.217/ seminar/19932033-1.pdf.
- Shah A, Garg A, Sairam K, Singh S. 2010. Pharmacological evaluation of eugenol loaded solid lipid nanoparticles in irritable bowel syndrome [Accessed 10 March 2011]. Available form: www.scientificipca.org/paper/2009/09/25/200909251910570A.do c.
- Chen F, Shi Z, Neoh KG, Kang ET. Antioxidant and antibacterial activities of eugenol and carvacrol-grafted chitosan nanoparticles. Biotechnol Bioeng. 2009;104(1): 30-9.
- Jadhav BK, Khandelwal KR, Ketkar AR, Pisal SS. Formulation and evaluation of mucoadhesive nanocapsules containing eugenol for the treatment of periodontal diseases. Drug Dev. Ind. Pharm. 2004; 30: 195-203.
- Wang JX, Zhang ZB, Le Y, Zhao H, Chen JF. A novel strategy to produce highly stable and transparent aqueous 'nanosolutions' of water-insoluble drug molecules. Nanotechnology 2011; 22(30): 1-7.
- Iannitelli A, Grande R, Di Stefano A, Di Giulio M, Sozio P, Bessa LJ, Laserra S, Paolini C, Protasi F, Cellini L. Potential antibacterial activity of carvacrol-loaded Poly(DL-lactide-coglycolide) (PLGA) nanoparticles against microbial biofilm. Int. J. Mol. Sci. 2011; 12(8): 5039-51.
- Kurian R, Arulmozhi DK, Veeranjaneyulu A, Bodhankar SL. Effect of eugenol on animal models of nociception. Indian J. Pharmacol. 2006; 38: 341-5.
- ICH-Guidelines Q2(R1). Validation of Analytical Procedures: Text and Methodology. Geneva, Switzerland: 2005